The resulting protein was resuspended in PBS with a protease inhibitor cocktail (Roche), and was then transferred to a 50 mL microtube. The cells were incubated for 2 min at 4 °C, followed by a corresponding Alexa Fluor-conjugated secondary antibodies (Supplementary Table 8) for 1 h. All mice were given a single intraperitoneal injection of Amuc_1409*. This was